In this present study, the bark extracts of Erythrina variegate were subjected to evaluation of the membrane stabilizing activity by using human erythrocyte and the results were compared with standard anti-inflammatory drug, acetyl salicylic acid (ASA). In vitro membrane stabilizing activity for hypotonic solution induced haemolysis, the ethanol extract inhibited 78.92% haemolysis of RBCs as compared to 85.42% produced by acetyl salicylic acid (ASA) and during heat induced condition different organic soluble materials of Erythrina variegate demonstrated 75.68%, 77.19% and 73.83% inhibition of haemolysis of RBCs respectively whereas ASA inhibited 79.04%.