An investigation has been carried out to evaluate the in vitro hepatoprotective effect of ethanolic extract of Coldenia procumbens Linn using antitubercular drugs as toxicant and silymarin as standard drug by MTT assay[(3-(4,5 dimethylthiazole –2 yl)-2,5 diphenyl tetrazolium bromide) assay.]Coldenia procumbens Linn has been widely used for a number of medicinal purposes especially in Siddha medicine. However, the information available on the pharmacological activity of the plant is very limited. Hence, it was proposed to carry out a preliminary in vitro analysis of the hepato protective activity of the plant, which gave promising results. This is the first report of the hepatoprotective activity of the plant