Nowadays, poor diet and stress of urban life cause many diseases and health disorders. Free radicals accumulation inside the body may be increased due to unsuitable diet and stresses in which most biochemical process is negatively affected. Due to the presence of a lot of antioxidant and radical scavenging compounds, the role of plant as a guarantor of human health is highlighted. Due to the biochemical properties of artichoke as a rich source of antioxidant compounds, the in vitro changes in antioxidant compounds namely phenols, flavonoids and radical scavenging properties of extract using methyl jasmonate and salicylic acid as stimulator were studied. The artichoke callus samples were transferred to a solid MS medium containing different concentrations of methyl Jasmonate and salicylic acid (0, 25, 50, 100, 200 μM). 28 days following subculture the biochemical compounds were investigated. Results showed that salicylic acid had significant effect on all measured parameters. The highest content of phenol and flavonoids of callus were observed in the samples in which treated with 200 μM salicylic acid. The highest radical scavenging percentage was obtained from the samples which were treated with 100 μM salicylic acid. Results showed that by increasing the amount of methyl jasmonate a reduction in the phenol compounds was observed. Contrary to that no clear trend was observed in flavonoid content. Based on the obtained results application of elicitors like Jasmonic and salicylic acids in artichoke media in certain amount stimulates may enhance the production of antioxidant compounds of callus extract.