The present study was designed to investigate phenolic content, antidiabetic and antioxidant activity of Morus nigra seeds. Extracts were prepared by soxhlet extraction using petroleum ether, ethyl acetate, ethanol and double distilled water as solvent in increasing order of polarity. The highest extractive yield was obtained in aqueous extract. Each extract was subjected to undergo phytochemical screening by standard protocol. Phytochemical screening revealed the presence of numerous bioactive secondary metabolites in seeds. Total phenolic content was estimated by Folin-Ciocalteau method. Ethanol extract contain highest phenolic content which was found to be 7.44 mg GAE/100 g dw. Antidiabetic activity was evaluated by α- amylase and α- glucosidase inhibitory assay while antioxidant activity was estimated by DPPH radical scavenging assay and ferric reducing antioxidant potential (FRAP) method. Ethanol extract demonstrated prominent α- amylase (IC50 = 679.50 μg ml-1), α- glucosidase (IC50 = 327.90 μg ml-1), DPPH radical scavenging (IC50 = 211.00 μg ml-1) and FRAP activity (278.64 μM/ml, FRAP value 0.779). The study provides a scientific proof for Morus nigra seeds regarding their traditional therapeutic application. Seeds can be used to treat diabetes and its associated secondary complications that arise due to oxidative stress.