Phytochemical analysis and fluorescence characters of leaf of Couroupita guianensis were investigated. The leaf extracts showed the presence of alkaloids, phenolics, flavonoids, saponins and tannins in the medicinal plant. The antimicrobial effect of selected Indian medicinal plant was evaluated on bacterial strains like Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and Leuconostoc lactis and the fungal strains such as Aspergillus niger, Aspergillus flavus, Rhizopus indicus and Mucor indicus. The solvents used for the extraction of plant were water and methanol. The in vitro antimicrobial activity was performed by well diffusion method. The antibacterial activities of leaf of Couroupita guianensis in successive different solvent were tested against gram +ve and gram - ve organisms as well as the fungus. The methanol extract of C. guianensis showed the broader spectrum of antibacterial and antifungal activity when compared with aqueous extract.