Formulation of silver nitrate and silver sulphadiazine is choice of drug in topical treatment of burns and related inflammation. Ancient silver based nanomedicine of Ayurveda is raupya bhasma (RB) used in treatment of different ailments but RB is still not explored to their anti-inflammatory activity. To consider this an attempt has been made to evaluate in-vitro anti-inflammatory activity of RB against denaturation of protein. Denaturation of tissue proteins is one of the well-documented causes of inflammation. Different concentration of RB was incubated with egg albumin in specified experimental conditions and subjected to determination of absorbance and viscosity to assess the in-vitro anti-inflammatory property using diclofenac sodium as standard against denaturation of protein. RB inhibited protein denaturation in dose dependent manner. The effect of RB as anti-inflammatory agent was found to be better than standard (diclofenac sodium) as the IC50 value of RB and diclofenac sodium are 43.2 μg/mL 46.1μg/mL respectively. Inhibition of denaturation of protein was further confirmed by change in viscosity. On the basis of present research it is concluded that RB possessed marked anti-inflammatory activity against the denaturation of protein