Plants are the basis of many of the modern pharmaceuticals used today for our various ailments. The aim of the present study is to evaluate the in-vitro antifungal activities of Croton Sparsiflorus plant extract in different organic solvents like hexane, chloroform, ethyl acetate and methanol. The antifungal activities of the extract were studied against the three fungal organisms like Aspergillus niger, Candida albicans and Candida tropicalis by agar well cut diffusion method. The results were compared with Ketoconazole which was used as a standard. All of the extracts exhibited varied antifungal activities at different concentration and the inhibition efficiency varied from solvent to solvent depending on the type of the species. The antifungal activities increased as the concentration of the extract increased. At the maximum concentration of 10 ml, the methanol extract of the root and stem showed the highest inhibition activity around 30 mm against Candida albicans and Candida tropicalis.