Objective: To evaluate the in vitro activity of anticancer (cervical cell line) and antimicrobial activity of ethanolic extract of acacia chundra leaves. Methods: The ethanolic extract of acacia chundra leaves were evaluated for in vitro anticancer cell line studies by MTT assay and antimicrobial activity by Disc diffusion assay. Results: Acacia chundra willd is one of the indigenous plants, which has enormous traditional values against various diseases. A phytochemical study revealed the leaves shows the presence of Alkaloids, Flavanoids, Tannins, Phenols and Steroids. The physiochemical analysis was done. Antibacterial and antifungal activity revealed that ethanolic extract of Acacia chundra was effective against against staphylococcus aereus, pseudomonas aerugenosa and candida albicans zone of inhibition was found to be 10 mm (250 μg), 18 mm (500 μg), 10 mm (250 μg), 20 mm (500 μg) and 16 mm (250 μg), 24 mm (500 μg) respectively. In vitro anti-cancer activity (Cervical cell line) was shown that the IC50 value of EAC was found to be 51.27 which prove plant shown moderate activity against cervical cell line studies. Conclusion: It is concluded that even though the accessibility of the modern system of medicine for simple and complicated diseases is available, many people in the studied area still continue to depend on medicinal plants, for the treatment of different type of diseases. Further the plant should explore for phytochemical responsible for anticancer and antimicrobial activity.