Being aware of the importance of wheat in the Moroccan economy, its protection against biotic stress mainly wheat septoria is essential asrespect of the environment. Thus, biological control is one of the most interesting long term strategies. In this frame, an in vitro study of antagonistic effects in direct confrontation was conducted between 5 antagonistic fungi and 5 isolates of Septoria spp. After incubation on PDA medium at 22°C, tests revealed that all antagonists could inhibit mycelia growth of these pathogenic isolates. Diametrical growth was reduced with percentages up to 84.21%, 52.43% and 37.36% respectively for antagonists from Trichoderma genus, for Talaromyces flavus and for antagonists from Acremonium genus. Similar results were obtained while performing remote testing confrontation but with smaller reduction percentages.