Pinitol is a very common cyclitol present in many leguminous plants and it is the mono methyl ether of d – inositol. The present study was designed to identify Pinitol, a proven anti-diabetic insulommetic drug in plants extracts by High Performance Thin Layer Chromatography (HPTLC). The TLC procedure was optimized with mobile phase chloroform: methanol: water (6:3.5:0.5) and ammoniacal silver nitrate as a derivatizing agent. The proposed HPTLC method was found to be simple, faster and specific laboratory method of identification of pinitol in plants extracts.