The production of aureofuscin is very low in the wild-type strain. We attempt to increase the production of aureofuscin by over-expression of a controlling gene in the wild-type strain. Recombinant bacterial strains were constructed by transforming SYAU0709 with an expression plasmid (pBJJ3M) that contained aurj3M, thereby increasing the number of aurj3M gene copies. The fermentation results showed aurJ3M gene could promote Aureofuscin production. Specifically, the recombinant strain produced approx. 600% more aureofuscin, as quantified by high-performance liquid chromatography analysis. The recombinant strains have the stable heritage.