Aegiceras corniculatum L. (Myrsinaceae) is a medicinally important mangrove occurring along the west coast of Maharashtra. A simple, sensitive, selective, precise, reliable and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of embelin has been validated for the determination of embelin from methanolic extracts of stem, leaves and fruit part of Aegiceras corniculatum L. embelin was quantified from these extracts using the solvent system of chloroform: ethyl acetate: acetic acid (5:4:1 v/v/v). This simple and precise method gave good resolution for embelin from other constituents of extracts at Rf 0.58. Further confirmation was done by LC-MS analysis in which embelin was observed at 295.1904 m/z ratio and retention time (RT) was 11.546 minute. We are reporting quantification of embelin from different plant parts for the first time.