Original Articles: 2016 Vol: 8 Issue: 8
Identification and Quantification flavonoids in three wild edible plants, Houttuynia cordata, Solanum gilo and Solanum kurzii of North-Eastern region in India, using High Performance Liquid Chromatography with Diode Array Detection
Abstract
Identification and quantification of flavonoids (aesculin, catechin, rutin ,naringin, myrecetin, coumarin, luteolin, quercetin, naringenin, apigenin and kaempferol) in two different solvent extracts (methanol and 80% aq. ethanol) of three wild edible leaves of viz. Houttuynia cordata, Solanum gilo and Solanum kurzii collected from Northeastern region in India has been performed using a reversed-phase high-performance liquid chromatography with photodiode array detector. The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm) , Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272 , 280 and 310 nm) using a mobile phase of acetonitrile and water with gradient elution . The experimental results showed good amount of catechin in the 80% aq. ethanol extract of H. cordata (6.63±0.046 mg/gm dry extract) and also in the 80% aq. ethanol extract of S. gilo (2.70±0.041 mg/gm dry extract). The high percentage of recovery (97-100%), low coefficient of variation ( R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of flavonoid compounds in the two plants under investigation.