In the present investigation, 2.0 M sodium benzoate solutions was employed as hydrotropic solubilizing agent to solubilize poorly water-soluble drug Tenfovir disoproxil fumerate for its spectrophotometric analysis. The primary objective of the present investigation was to employ these hydrotropic solutions to extract the drugs from their dosage forms, precluding the use of costlier organic solvents.Ultraviolet absorption spectrophotometric method for the estimation of poorly water soluble drugs like Tenfovir disoproxil fumerate in pharmaceutical formulations has been developed. Aqueous solubilities of this selected model drugs was to a great extent (112 to 121 fold) in 2.0 M sodium benzoate solutions. The selected
max for Tenfovir disoproxil fumerate were 317 nm respectively. The hydrotropic solutions used did not show any absorbance above 332 nm, and therefore, no interference in the estimation was seen. The results of analysis have been validated statistically, and by recovery studies.