Bifunctional Pt/H-Beta and Pt/H-Mordenite catalysts were prepared by using incipient wetness impregnation method to obtain a 0.5 wt% metal load and applied to the Isomerization of nhexane. Commercial Pt/H-Beta gave rise to a higher yield of high octane Dimethylbutanes (DMB) than the commercial Pt/H-Mordenite. To explain this result, both the dispersion and distribution of Pt clusters in the zeolite channels were investigated. The acidity and distribution of acid sites were examined by using ammonia and pyridine as probe molecules. Pt/H-Beta was found to be a potential catalyst for the Isomerization of n-hexane because Pt clusters could be well dispersed to give proper balance between metallic centers and acid sites in H-Beta zeolite although its acidity was low as compared to H-Mordenite.