Isolated chroman derivative from the seeds of Ensete superbum Cheesm have been reported to possess anti-implantation activity. The literature survey reveals that no HPTLC methods are reported for the present drug. Hence, an attempt wa s made that the active derivative can be selected as a standard for the presence of same in different fractions respectively. The isolated chroman derivative along with the fractions was sub jected to multi-wavelength scanning and spectrum analysis. The detection and quantification were observed at wavelengths of 256 and 550 nm. HPTLC analysis indicated that the maximum a mount of isolated derivative was present in ethanol fraction (1.83%) followed by ethyl acetate (1.74%) and methanol frac tion (0.70%).The petroleum ether fraction of the extract was found to contain no traces of the isolated compound. Linearity studies indicated that isolated derivative was present in the linear range of 100 -500 ng. Since this method resolves and quanti fies the isolated chroman derivative effectively, therefore it can be used as a marker c ompound to quantify the concentration of this active principle in its different extracts