Digera muricata (L.), Family Amaranthaceae, wild edible plant commonly known as ‘latmahuria’. It is commonly distributed throughout the India. In Ayurveda, the herb is considered as a cooling, astringent to the bowels and also used as laxative. The flowers and seeds are used to treat urinary discharges.Hence, Digera muricata (L.) Mart. is used in both folk and traditional system of medicine. In the present study, H.P.T.L.C analysis and antioxidant [2,2- diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] activity of methanolic extract of whole plant of Digera muricata were investigated.The methanolic extract was obtained by soxhlation for 72 hrs. The methanolic extract showed elevated DPPH scavenging activity, and had a promising activity which increased with concentration. A positive correlation was established between the level of phytoconstituents and antioxidant activities.