Urine from the pregnant women was collected and then subjected to estimate the protein content which was found to be 243.73 mg with a biological activity of 50IU/mg. Since, purity of a protein has been directly proportional to its biological activity, the obtained protein HCG was subjected to the solvent precipitation method. Subsequently, the purity increased to 150 IU/mg in content of 81.24 mg. After the salt fractionation method, the protein content 48.89 mg exhibited 250 IU/mg of biological activity. This led to the extraction of crude HCG of 24.36 mg with a biological activity of 500 IU/mg.