The aim of this research is to determine the power or ability catalytic heterogeneous catalyst chitosan derivatives are carboxy methyl chitosan-urea-glutaric acid (CMChi-UGLU) and 2,2-dichloro-carboxy methyl chitosan-urea pthalic acid (DCMChi-UTER) on the reaction of trans- esterification palm kernel oil (PKO) -methanol with fluidization method. In this research showed that both the catalyst may change in PKO triglycerides into methyl ester are methyl laurate, methyl caprate, methyl miristate, methyl palmitate, methyl oleate, methyl linoleate, and methyl stearate. The trans-esterification PKO-methanlo with CMChi-UGLU as catalyst give a total fatty acid methyl ester C14-C24 (FAME) is 32.62% and conversion value 36.28%, while DCMChi-UTER as catalyst give total FAME is 11.93% and conversion value is 3.24%.