Root nodules are highly organized root organs where the nitrogen fixation take place in, its formation are the results of complicated interactions between legumes and rhizobia. The nodule formation and nitrogen fixation are energy-demanding processes. During nodule formation and nitrogen fixation，a large amount of sucrose, as the major end product of photosynthesis is required to be transported into nodules. In legume root nodules, hydrolysis of sucrose by Sucrose synthase (SuSy) is a necessary prerequisite for normal nodule development and a key step in nitrogen fixation. Deficient SuSy activity in nodules renders them incapable of effective nitrogen fixation. ENOD40 play a role in the regulation of sucrose utilization in nodules through the soyabean ENOD40 peptides directly binding to SuSy.In both legumes and non-legumes, ENOD40 expression is important in nodule organogenesis and development. However, during symbiotic development, whether Nod factor signaling associate with in the regulation of sucrose utilization in nodules is unknown. NORK the immediately downstream component of these Nod factor receptors, is central to the Nod factor signalling cascade. NORK functions not only in the early signaling pathway operative in root hairs, but also in later stages of nodule formation. In this study, we found that the GmENOD40expression level decreased in GmNORKRNAisoybean transgenic root by rhizobial inoculation. Thus provide important detail information toward understanding the functions of NORK and GmENOD in symbiotic signaling and nodule development.