Objective: Present study screening of soil actinomycetes near herbal plant area in Girnal mountain region of Junagadh district of Saurashtra Gujarat in respect to its antimicrobial activity and biochemical characterization of active isolates. Methods: 11 different herbal plant sites selected randomly from Girnar mountain area. Soil collected from microflora part of soil depth up to 30 cm with sterile articles used. Samples treated and serially diluted for good colony result. Different growth and ISP media tested for colony study and make pure culture using microbial method. Morphological and microscopic study was done of colonies. Antimicrobial activity checked by cross streak method tested against various test pathogens. Secondary screening was done by agar well diffusion method. Various biochemical testes for characterization were done of active isolates. Results: 23 isolates of actinomycetes were observed with dominant arial mycelium white color in 56.52% of isolates and 43.48% yellow color in vegetative mycelium. 13 (56.52%) isolates were classified and belongs to streptomyces genus. More than 20 mm ZOI (Zone of inhibition) observed in 3 isolates (against Bacillus subtilis), 4 isolates (against Staphylococcus aureus), 2 isolates (against Proteus vulgaris), 2 isolates (against Escherichia coli) and 2 isolates (against Klebsiella aerogenes). JRI1, JOS1, JAC1, JTF1 and JPC1 were observed most active isolates. Conclusion: Out of active isolates range of ZOI was between 15 to 20 mm is most frequently observed. 11 isolates showed activity and 12 isolates not showed any activity against test pathogens. Soil actinomycetes near some medicinal plant area of Girnar forest sites contain actinomycetes which have antagonistic activity. As Girnar forest was very rich source of herbal medicinal plants we correlate soil actinomycetes screening study for antimicrobial activity samples taken near plant area and these ecological sites had potential to produce new antimicrobial compound with other metabolites from those actinomycetes.