In this study, the bioactive components of Trigonella foenum grecum seed have been evaluated using GC/MS. The chemical compositions of the hydroalcholic extract of Trigonella foenum grecum seed were investigated using Perkin-Elmer Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC/MS analysis of ethanol extract of Trigonella foenum grecum seed revealed the existence of á-D-Glucopyranoside, methyl (74.54.00%),3-OMethyl- d-glucose (16.11%)2-Propen-1-amine, N-ethyl- (3.43%), Aziridine, 1,2,3-trimethyl-, trans- (2.41%),. The results of this study offer a platform of using Trigonella foenum graecum seed as herbal alternative for the current synthetic antimicrobial agents