Secondary metabolites and different phytochemicals are present in a great deal in the plant Kalanchoe pinnata belonging to family Crassulaceae. However, few reported the scientific data regarding GC-MS analysis of methanolic extract of Kalanchoe pinnata fresh leaves till date during literature review. The study was aimed to verify the phytochemical in the methanolic extract of Kalanchoe pinnata fresh leaves. Methanolic extraction of fresh leaves Kalanchoe pinnata was performed and GC-MS analysis data was provided by IIT, Powai, Mumbai. Phytochemical investigation was carried out to identify the possible components from Kalanchoe pinnata fresh leaves by GC-MS. It has revealed only major three peaks of GC-MS spectrum that phytol, squalene and 5- oxotetrahydrofuran-2,3-dicarboxylic acid dimethyl ester. Other two phytocomponents remained unidentified and still reported no activity. Squalene is generally reported for anticancer, antioxidant and additional activities. From the results, it is firstly reported that phytol and squalene present as major bioactive compounds in methanolic extract of Kalanchoe pinnata fresh leaves.