Background and objective: Detecting the levels of liver function enzymes in serum is not sufficient to recognize liver injury status or the degree of liver damage. So tracing hepatic enzymes in tissue may be more accurate in diagnosing the case. Taurine and spirulina were selected in this study to test their therapeutic efficacy on hepatocytes mainly suffering from diabetes. Materials and methods: Rats were divided into five groups. 1-The control group. 2- The diabetic non treated group injected streptozotocin (STZ) 45 mg/kg b.wt. 3-The diabetic group treated with spirulinaplatensis (15 mg/kg.bd.wt). 4- The diabetic group treated with taurine (500 mg/kg.bd.wt).5-The diabetic group treated with the anti-diabetic drug; amaryl (0.15 mg/ kg.bd.wt).The study tested the influence of STZ (45 mg/kg b.wt) on the ability of the hepatocytes to biosynthesize aminotranseferases and lactate dehydrogenase enzymes in addition to the detection of the effect of the chosen dose of STZ on DNA status by using the comet assay in liver cells of diabetic and treated rats. Also the liver total protein content was evaluated in all the groups. Results and conclusion: Non significant changes in enzyme levels were observed in the liver tissues of the diabetic group treated with spirulina or amaryl drug compared to the diabetic non treated group. On the other hand, taurine treated group showed significant changes in the tissue enzymatic assays. The DNA comet assay of the liver tissue in did not show significant changes in all groups. The study concluded that taurine is suggested as an integrated treatment to compensate the missing targets of the anti-diabetic drugs.