The main objective of this work was to design, synthesized and evaluates the Floating microsphere of muscle relaxant drug Thiocolchicoside. Thiocolchicoside (THC) is used clinically for its muscle relaxant, anti-inflammatory, and analgesic properties. Microspheres are solid spherical particles ranging in size from 1-1000 μm. They are spherical free flowing particles consisting of proteins or synthetic polymers. They are biodegradable in nature. The work investigated the design and evaluation of microspheres by Ionotropic gelation technique method. The surface morphology study by SEM indicated that microspheres were spherical with smooth surface. There was no interaction between the drug and polymers, as studied by FTIR study. The prepared microspheres were characterized by entrapment efficiency, particle size, and micromeritics properties. The drug must be delivered for a prolonged period and many medicines must be taken simultaneously in case of chronic patients. The Floating microspheres were shown to be effective for increasing the bioavailability. Among all formulations, F5 showed better drug release rate and buoyancy, which is considered as the best formulation. Stability studies shows about less than 0.5 % of drug degrade in 60 days indicating relatively good stability study of the formulation. The Floating microspheres were shown to be effective for increasing the bioavailability of Thiocolchicoside in gastric region. From the current study it can be concluded that the increase in bioavailability due to the floating microsphere drug delivery system.