Aspergillus niger is saprophyte fungi that live in organic material such as corncob. A. niger was then isolated from corncob to obtained natural pigment from A. niger. The fermentation process was done using corncob as medium fermentation (liquid medium). The result showed tha t the optimum condition of fermentation of A. niger was of 8 days after cultivation with temperature was of 30 0 C and agitation condition of 150 rpm. Further, char acterization of natural pigment produced by using Thin Layer Chroma tography (TLC) found that there are two compounds w ith Rf 0,61 and 0,5.