The triumph of quantum dots (QDs) in biomedical applications roused the scientists to further extensive research in this area. Citrate stabilized zinc oxide QDs are useful in drug and gene surface loading. Response surface quadratic models are well reported to study the influence of independent parameters on dependent parameters. The objective of the present study was thus to carry out evaluation of citrate stabilized zinc oxide QDs by employing a statistical experimental design. From the results it was concluded that the particle size, polydispersity index and zeta potential data does not fit into the response surface quadratic design model. A comparison of the prepared citrate stabilized zinc oxide quantum dots was done with respect to particle size, polydispersity index and zeta potential. Though a response surface statistical modeling was not possible with the selected range of independent factors in the study, the results could be of use in choosing constraints for independent variables in further studies towards tailored citrate stabilized zinc oxide QDs with desired values for particle size, polydispersity index and zeta potential.