The present investigation was carried out to extract natural pigment from the fruits of Basella alba plant. The dye was extracted with methanol and then centrifuged at 10000 rpm and the supernatant was taken for analysis. The Basella alba fruit dye was used for dyeing the scoured cotton cloth as such and also using mordants like alum, FeSO4 and CuSO4. The dye component was extracted and dyeing of cotton was also studied. The spectroscopic characteristics of natural dyes on fabrics and also with the biopolymer carboxymethyl cellulose were also determined. The effect of pH on the extracted dye was studied and it was found that at different pH range dyed fabrics have different color. The interaction between natural dye and cationic surfactant like N-Cetyl Pyridinium Chloride monohydrate (NCPC) & anionic surfactants like sodium dodecyl sulphate (SDS) was also done for improving the dyeing process in respect of theoretical, technological, ecological and economical points of view. It is found that pigments from Basella alba fruits was moderately applicable in dyeing of cotton fibre as well as other fibres also. These findings also suggest that Basella alba is a rich source of betalains and could have potentiality for use in the development of food colorants, cosmetics, paper coloring, use as acid-base indicator and nutraceuticals in future.