Extraction of Mn(II) from an acetic acid-acetate buffer medium was investigated using D2EHPA and Cyanex 272 in kerosene. The effect of different parameters such as equilibrium pH, extractant concentrations, metal ion concentration, sodium acetate concentration and temperature on the extraction system was studied. The percentage extraction of manganese was 96% with 0.1M D2EHPA corresponding to equilibrium pH 4.52 whilst it was 76% with 0.1M Cyanex 272 corresponding to equilibrium pH 4.86. Quantitative extraction of manganese was achieved with 0.8M D2EHPA. The loading capacities of both the extractants were found out. On the basis of slope analysis results, the species extracted into the organic phases were proposed to be MnA2.2HA and MnA2.4HA for both D2EHPA and Cyanex 272.When a ternary mixture containing 0.02M Mn(II), 0.01M Cu(II) and 0.01M Co(II) was extracted with D2EHPA, the order of extraction of metal ions is Mn(II)>Cu(II)>Co(II). Highest separation factors of 52.5(βMn⁄Cu) and 280.3(βMn⁄Co) was obtained from aqueous solution having pH 5.0 and extractant concentration of 0.05M D2EHPA in kerosene.