This paper presents an approach to remove the dye using chitosan coated glass beads at various pH, temperature and contact time. Dye decolorization using biopolymers are recent method of interest to remove the dyes from the waste water. In this initial attempt, the chitosan was extracted from prawn shell wastes and applied in bio sorption of azo dyes. This polymer was encapsulated over glass beads. The encapsulated glass beads were packed in a glass column and azo dye decolorization was carried out and determined calorimetrically using UV-Visible spectrophotometer and different isotherms were calculated. The isotherms such as Langmuir, Freundlich and Temkin were examined and the appropriate model was identified. The interaction of the dye with chitosan was studied using SEM and FTIR.