Herbal medicines are the major remedy in traditional medical systems from thousand of years and made a great contribution in maintaining human health and in preventing many infectious diseases. Ayurvedic herbal preparations based on the medicinal plants are used in modern health care to prevent common bacterial diseases but their antibacterial potential was not evaluated in detail. Therefore, an effort was made to evaluate the antibacterial potential of herbal Ayurvedic product against enteric bacterial pathogens. The antibacterial potentials of some herbal preparations used in Ayurvedic system of medicine namely Ashwagandhadi churna, Bilwadi churna, Chandanadi churna, Chopchinyadi churna, Dashmula churna, Dhatupaushtic churna, Eladi churna, Gangadhar churna, Hingwashtak churna, Jatiphaladi churna, Lavangadi churna, Kutuja churna, Narayan churna, Sarswat churna, Shatawaryadi churna, Talisadi churna and Shivkshar Pachan churna were investigated by disc diffusion method against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus epidermidis, Salmonella typhimurium, Proteus vulgaris. The study in vitro corroborated the antibacterial potential of the ayurvedic preparation used in Indian system of medicine and showed antibacterial activities against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Staphylococcus epidermidis and Proteus vulgaris. Thus study showed that these herbal preparations might be alternative medicine to combat enteric bacterial infection in human being.