This study was performed to find out the relationship between the adipocytokines and antioxidant vitamins in the development of type 2 diabetes mellitus (T2DM). 45 subjects divided into 3 groups (15 for each group): normal subjects, diabetic subjects, obese diabetic subjects. Serum glucose, lipid profile, insulin, adipocytokines including (leptin, adiponectin, resistin) and antioxidant vitamins (A&E) were estimated. The mean values of Serum insulin and leptin levels were significantly higher in obese diabetics while significantly lower in diabetics compared to the control group. In contrast, adiponectin concentration decreased significantly in diabetic and obese diabetic groups when compared to the control. No significant difference in serum resistin level between the three groups. There was a significant decrease in vitamin A level in both diabetic and obese diabetic groups as compared to the control group. Whereas, there was none statistically significant decrease in serum level of vitamin E in both II & III groups comparing to controls. These results indicated the importance of taking these adipocytokines in consideration as biomarkers for T2DM and obesity during treatment. We suggest the inclusion of dietary supplementation of the antioxidant vitamins A & E in the management of type 2 diabetes mellitus.