The paper aims at verifying the influence of supplemented spirulina on the form of erythrocytes and the content of hemoglobin of anaemic ICR male rats that have done exercises. The method adopted: scanning electronic microscope and full automated hematology analyzer are used to studying the influence of supplementing spirulina on the form of erythrocytes and the content of hemoglobin of rats. Results: The malform ratio of the erythrocytes of ICR male rats in the group doing exercises is significantly higher than the control group, and the group supplemented with spirulina (P<0.01); the content of hemoglobin of ICR male the rats from the exercising group is significantly lower than that from the normal control group and the group supplemented with spirulina (P<0.01); the content of hemoglobin of ICR male rats from the exercising group is significantly lower than that from the normal control group and the spirulina group (P<0.01) ; the content of hemoglobin ofICR male rats from the spirulina group is lower than the normal control group (P<0.01). Conclusion: Spirulina can significantly reduce the malform ratio of erythrocytes of ICR male rats after doing exercises, and improve the content of hemoglobin of ICR male rats after doing exercises; this paper provides an experimentation basis for application and development of spirulina in the field of exercises.