This paper aims at studying the influence of Morchella esculenta Morchella esculentaMorchella esculenta Morchella esculenta Morchella esculenta Morchella esculenta on nitrogen monoxide and NOS system in the rats that have done high strength durability exercises. Experiment is conducted for comparison by feeding exopolysaccharide extract to wistar male rats that have done high strength durability exercises to observe the change in the content of NO and NOS in different issues of wistar male rats that have done high strength durability exercises. Result: the content of NO in the liver tissue of the group that has done exercises and taken drugs is significantly lower than the group that has done exercises (P<0.05); the total activity of NOS enzyme in the cardiac cardiac cardiac cardiac cardiac muscle muscle muscle muscle tissue of the group that has done exercises and taken drugs is lower than the group that has done exercises, but the difference is insignificant (P<0.05); the total activity of NOS enzyme in the liver tissue of the group that has done exercises and taken drugs is lower than the group that has done exercises, but the difference is insignificant (P>0.05). Conclusions: the content of NO in the cardiac muscle cardiac muscle cardiac muscle cardiac muscle cardiac muscle cardiac muscle cardiac muscle tissue and the liver tissue in the rats can be changed by supplementing exopolysaccharide extract to improve the constriction of blood vessels in the hearts and the livers of rats having done exercises to stretch blood vessels and increase blood supply.