Information system plays an important role in the development of regional tourism cooperation, and the mature application could avoid the benefits loss in regional tourism cooperation. By the analysis of the online information system, the communication between regional cooperation organizations is broader, while the organizations are operated more efficiently and smoothly. The findings are that evolution process of regional tourism cooperation organization includes four stages, regional tourism cooperation organization led by the government, regional tourism cooperation organization with the structure of “star topology”, regional tourism cooperation organization with functional modular, and network regional tourism cooperation organization