This study aimed at evaluating the protective role of Se-Y against TAA induced hepatotoxicity. Sixty-four adult female rats were divided into 8 group: (1) served as control, (2) was administered TAA, (3 and 4) were given two dose levels of Se (0.95 and 0.47 mg kg-1 day-1) respectively with simultaneous administration of TAA, (5 and 6) were given two dose levels of yeast (66.5 and 33.25mg kg-1 day-1) respectively with simultaneous administration of TAA and (7 and 8) were given two dose levels of Se-Y (66.5 and 33.25mg kg-1 day-1) respectively with simultaneous administration of TAA. Serum ALT, AST, ALP activities, TNF-α, MCP-1, IL-6, IL-8, laminin and fibronectin levels were determined. Histopathological examination of liver tissue sections was carried out. The results revealed that TAA intoxication caused significant increase in serum ALT, AST, ALP activities, TNF-α, MCP-1, IL-6, IL-8, laminin and fibronectin levels versus the control. Histopathological investigation of liver tissue sections of TAA intoxicated rats showed necrosis with inflammatory cell infiltration. Co-treatment with Se, yeast or Se-Y produced significant protection against TAA-induced hepatotoxicity as indicated by marked improvement in the studied biochemical markers. In conclusion, Se, yeast and Se-Y could protect the rat liver from TAA induced hepatotoxicity.