This work aims to investigate the in vitro antioxidant activity of two ferrocene derivatives: 2-nitrophenylferrocene and 4-nitrophenylferrocene using different assays, including DPPH radical scavenging assay, phosphomolybdenum reduction assay and superoxide anion radical. The results indicated that 2-nitrophenylferrocene has the most important percentage of scavenging DPPH radical with IC50 = 0.48 mg/ml, IC50 of total antioxidant capacity (TAC) evaluating by phosphomolybdenum assay of 26.323 mg/ml, 4-nitrophenylferrocene has the highest TAC estimated by the decrease of the current density of anodic peak of oxygen with 10.907 and the highest scavenging activity of superoxide radical with IC50 = 1.16 mg/ml.