The Root extract of Agavesisalana was tested as a larvicide against three vector mosquito species viz., Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti of the Root extract at 2% dilution produced 100% mortality of III rd instar larvae of An. stephensi and 1% dilution produced 100% mortality in case of Cx. quinquefasciatus and Ae. aegypti. For further bioassays, the LC50 and LC90 value of methanol and petroleum ether extracts of Sphaeranthus indicus Roots were determined against instar larvae of An. stephensi, Cx. quinquefasciatus and Ae.aegypti and these values were 125, 62.5, 31.25, 15.62 & 7.81ppm respectively. The present study revealed that Sphaeranthus indicus Root extract possess larvicidal activity against Cx. quinquefasciatus, Ae. aegypti and An. stephensi and can be exploited for their control under integrated approach of vector control.