The seeds of Celastruspaniculatuswere collected and pulverized to fine powder and the powder was extracted by Soxhlet (continuous Hot Extraction) apparatus using methanol as solvent. In-vitro studies were carried out using various reagents like Ferrous Sulphate, Potassium thiocynate, Ferrozine, Desferrioxamine, purified water and methanolic extract of crude drug. The % iron-inhibition of standard and test drugs were measured. Animal studies were done using Iron intoxicated rats and Albino Wistar rats were subjected for iron dextrose injection (i. p.) six times in 21 days to all the groups (except normal control) and after treatment duration, Serum iron level, SGOT, SGPT, CKMB & Creatinine levels were measured. The iron chelating activity of Celastruspaniculatus was confirmed by in-vitro evaluation. There was significant decrease in serum iron levels in test group as compared to disease control group animals. The organo-protective effects on liver, heart and kidney was confirmed by reduction in various bio-markers like SGPT, SGOT, Creatinine and CKMB levels as compared to iron overloaded rats. The results of the study has shown that the methanolic extract of Celastruspaniculatus have significant iron chelating activity. It also has beneficial effects on hematological parameters and organo-protective effects were observed during study. So it can be useful in the management of iron overload disorders like thalassemia and hemochromatosis like conditions.