In current perspective of time there is a need to develop new compounds having great therapeutic potential. In the present research work different antioxidant and thrombolytic activities of sulfonamide derivative synthetic compounds were screened out. Sulfonamides are enthusiast to a biologically very important category of compounds and are under consideration of many pharmacologists and synthetic chemists. By keeping in view the prominence of sulfonamide derivative compounds were tested for their antioxidant properties through DPPH free radical scavenging abilities and oxidative DNA damage protection assays. It was observed that all the tested compounds showed good to moderate antioxidant potential. Similarly, the thrombolytic effect was screened out through in vitro clot lysis activity which indicated that such synthetic derivatives have biological potential and could be integrated in drug formation for achievement of therapeutic goals.