Natural products particularly plant origin have played a vital role in prevention and mitigation of human diseases. The present study was carried to evaluate the anti-hyperglycemic and wound healing activity of the leaf extracts of Tiliacora acuminata (Lam) on rats, the aqueous leaf extract was found to produce significant reduction of blood glucose concentration between 2-4 h of administration in alloxan induced hyperglycaemic rats at tested dose levels. However, in normoglycaemic animals, the extract at 400mg/kg produced significant reduction of blood glucose between 2-4 h of administration. In the wound healing studies, the leaf juice was found to be more active than the extracts.