In the present study an attempt was made to evaluate the anxiolytic activity of ethanolic extract of Pisonia grandis R.Br leaves (EEPGL) in mice. The anxiolytic activity was evaluated by Elevated plus maze, Y-maze, Hole-board and Actophotometer behavior models. The efficacy of the extract (150 and 300 mg/kg, p. o) were compared with the standard drug Diazepam (2 mg/kg, p. o). EEPGL showed significantly increased the number of entries and time spent in the open arm in the elevated plus maze, increased the number of head dipping and line crossing in Hole board, decrease in locomotor in their respective Behavior Models. This study provides scientific basis of anxiolytic activity of Pisonia grandis R.Br.