Diabetes mellitus (DM) is an endocrine disorder characterized by abnormal carbohydrate, lipid and protein metabolism along with specific long-term complications which are associated with oxidative stress (Pandey A, 2011). Hence, it is important to discover a hypoglycemic drug that reduces oxidative stress in diabetic patients. This study, therefore, was performed to investigate the antioxidant potential of Pioglitazone (PIO) by nitric oxide scavenging activity, an in –vitro method. Pioglitazone antioxidant property was analyzed with varying concentration from 100 to 1000 μg/ml using spectrophotometer while keeping ascorbic acid as the standard. The results of this study show that Pioglitazone, on comparison with Ascorbic acid, has dose dependent antioxidant property.