Medicinal plants are finding use as pharmaceuticals , nutraceuticals, cosmetics and food supplements. Even as traditional source of medicine s and they continue to play pivotal rule. Ethanolic extract of three medicinal plants viz ., Acalypha indica, Ricinus communis and Euphorbia hirta were evaluated for there therapeutic potential as antimicrobial agent against six standard organisms 3 bacteria (Klebsiela pneumoniae, Salmonella typhi, Pseudomona s aeruginosa ) and 3 fungi ( Aspergillus flavus, Fusarium oxysporium, Penicilliu m chrysogenum ) by using Filter Paper Disc Method. Antimicrobial effi cacy of callus cultures of E. hirta with inhibition zone of 16 mm was found to highest again st F. oxysporium (Fungus) and in A. indica with inhibition zone of 27 mm was found to maximum against K. pneumoniae (Bacteria). Contrary, in in vivo analysis of antimicrobial efficacy of A. indica with inhibition zone of 15 mm were found to highest against P. chrysogenum (Fungus) and E. hirta with inhibition zone of 25 mm was found to highest against K. pneumoniae (Bacteria).