Antimicrobial efficiency of Jasminum sambac aromatic plants leaf extracts were examined using petroleum ether, chloroform, ethyl acetate and ethanol as solvents and tested against eight human pathogens like Bacteria: Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Fungi: Aspergillus niger, Aspergillus flavus, Candida albicans using agar disc diffusion method. The ethanol extracts of Jasminum sambac showed highest antibacterial activity against than that moderate the ethyl acetate, petroleum ether and chloroform the bacterial strains tested. The mean zone of inhibition produced by the extracts in disc diffusion assays were ranged from 5 mm to 27 mm. All the plants showed significant activity against all pathogens. The minimum zone of inhibition and comparatively greater inhibitory concentration were determined in petroleum ether and chloroform extract of Jasminum sambac showing less antimicrobial activity against all the experimental strains. The preliminary phytochemical analysis of presence and absence of different solvent extracts of Alkaloid, Flavonoid, tannin, Saponin, glycoside, steroid and terpinoid. The Spectrum of activity observed in the present study may be indicative of the present study ethanolic extracts of these plants could be a possible source to obtained new and effective herbal medicines to treat infections, hence justified the tribal uses of Jasminum sambac against various infectious diseases.