In the last decades of the nineteenth century, the study of disease – causing microorganisms became concentrated on bacteria and largely institutionalized. In earlier years, scientists interested in bacteria had originally been chemists like Pasteur, physicists like Tyndall, or Botanists like Cohn and Ward. For this reason, the objective of this research was to evaluate the potential of some dithiolethiones on standard microorganism strains as well as multidrug resistant bacteria, which were isolated from hospitals. Recent studies have demonstrated that two dithiolethiones compounds, particularly (4-phenyl-1,2-dithiole-3-thione), exhibit the biological activity against Staphylococcus aureus. Antibacterial activity showed value ranged from 1.02 to 1.428 mg/ml (MIC). All of the tested dithiolethiones and antibiotics were inactive against Escherichia coli and Pseudomonas aeruginosa, except Amikacin (30μg) which showed the highest antibacterial effect against all tested bacteria.