Tabernaemontana is genius of flowering plant in the family of apocynacea, plant is rich in indole alkaloids and parts of the have been used to relieve pain in some parts of Asian countries. In present study from the methanol extract of Tabernaemontana sananho leaves two new alkaloids compounds were isolated and structure was elucidated by spectral studies as TS-01, a subtype of ervatamine and TS-02 belonging to monoterpene indole alkaloid. Methanolic extract of Tabernaemontana sananho leaves was evaluated for anti inflammatory and antinociceptive activity at dose of 150 mg/kg and 300 mg/kg respectively, and a significant activity dose dependent (p< 0.001) activity was exhibited, confirming the folk- fore use of Tabernaemontana sananho in treating inflammation .