The present study was aimed to screen Cassia obtusifolia (CO) seeds for anti-inflammatory potential using carrageenan-induced paw oedema (Acute model) and cotton pellet induced granuloma (sub-chronic model) models in Wistar rats. In the study, 90% methanolic extract (ME) of the seeds was prepared and studied in carrageenan-induced paw oedema model at three different dose levels: 125, 250 and 500 mg/kg, orally. The ME (500 mg/kg) showed significant (P<0.05) anti-inflammatory activity in this model therefore, selected for further evaluation in sub-chronic model. The results of ME (500 mg/kg) were at par with diclofenac sodium (20 mg/kg). The study shows that CO seeds have anti-inflammatory potential and can be studied further to find out the compound responsible for the activity.