The present study was design with an aim to evaluate the antidiabetic potential of Momordica balsamina (MB) seeds. The study was performed on methanolic and aqueous extracts of MB seeds in oral glucose tolerance test (OGTT) and STZ-induced diabetes models in rats. In OGTT model, aqueous extract (AE) at 500 mg/kg dose had shown the significant (P<0.05) check in the rise of blood glucose, at par to metformin (500 mg/kg). Hence, AE (500 mg/kg) was selected for further study in STZ-induced diabetes model. Single dose of STZ (45 mg/kg i.p.) was used for diabetes induction, which had shown significant rise in blood glucose level, and significant decrease in body weight as compared to normal control rats. Three weeks treatment of diabetic animals with AE (500 mg/kg) showed significant check in rise of blood sugar compared to untreated diabetic rats along with improved complete lipid profile and body weight. On the basis of analysis of data obtained during the study, it may be concluded that AE of MB seeds is having significant antihyperglycemic potential and can be further fractionated in order to get a responsible molecule for this vary action.