The main aim of this study is to determine the antibacterial and antifungal activities of ethanol and hexane extracts of Wedelia chinensis leaf against both pathogenic bacteria and fungi by agar disc diffusion method. Both the extract showed significant antibacterial activities against four gram-positive (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus) and four gram-negative (Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli) bacteria. The antifungal assay was done against four fungi (Aspergillus niger, Aspergillus flavus, Candida albicans, Alternaria alternata). For all the cases it showed significant result. Thus this plant can be used as phytomedicine to treat the bacterial and fungal diseases.